Module manager: Chiara Gorrini
Email: C.Gorrini@leeds.ac.uk
Taught: Semester 1 (Sep to Jan) View Timetable
Year running 2024/25
This module is only available to Masters students in the Faculty of Biological Sciences. BSc in Biological Sciences and knowledge of cell biology.
BIOL5112M - Bioimaging BIOL5312M - Bioimaging
This module is not approved as an Elective
The aims of this module are:
- To gain theoretical knowledge of a wide range of imaging techniques applicable in modern cell biology.
- To gain some practical experience of preparing, fixing and staining cells for visualisation of the nucleus and cytoskeleton
- To gain some practical experience in imaging cellular organelles in live cells.
On completion of this module, students should be able to:
1. Describe the principles of phase, DIC, fluorescence, deconvolution and confocal microscopy, calcium imaging, and transmission and scanning electron microscopy;
2. Perform the techniques required to obtain epifluorescence, confocal and deconvolution images of fluorescently stained fixed cells;
3. Explain how to image live cells with an epifluorescent or confocal microscope;
4. Explain the principles of the techniques FRAP, FRET and FLIM microscopy;
5. Understand the principles of imaging cells, and single molecules in the electron microscope;
6. Develop and understanding of how to analyse imaging data
1. Writing an essay
2. Writing a practical report
3. Ability to present imaging data in a practical report
The aim of this module is to provide an introduction to the wide range of approaches used in Bio-imaging and their relative advantages and disadvantages for analysing protein and cellular function. Bio-imaging encompasses a wide range of approaches from imaging single molecules in the electron microscope, to determining the localisation of proteins in fixed cells by fluorescence microscopy. Any one, or a combination of techniques can build up a picture of the structure and function of a protein of interest and its biological role in the cell.
The module will include a range of lectures/seminars on the techniques/approaches involved together with practical demonstrations and practical work to enable the student to obtain hands on experience of some of the techniques. The practical work will be based on visualising components of the cellular cytoskeleton (actin,and microtubules) and investigating organelle dynamics and protein localisation in plant cells.
| Delivery type | Number | Length hours | Student hours |
|---|---|---|---|
| Lecture | 6 | 2 | 12 |
| Practical | 1 | 2 | 2 |
| Practical | 5 | 3 | 15 |
| Private study hours | 121 | ||
| Total Contact hours | 29 | ||
| Total hours (100hr per 10 credits) | 150 | ||
By attendance at practical classes and tutorials.
| Assessment type | Notes | % of formal assessment |
|---|---|---|
| Literature Review | 2,000 words | 50 |
| Report | 2,500 words | 50 |
| Total percentage (Assessment Coursework) | 100 | |
Normally resits will be assessed by the same methodology as the first attempt, unless otherwise stated
The reading list is available from the Library website
Last updated: 8/2/2024
Errors, omissions, failed links etc should be notified to the Catalogue Team